Cell+Investigations


 * __Cell Invesitgations in topic 2__**

Title: Cell Growth (pp.130 - 131 for stages of mitosis)
Aim: To determine whether root cell growth occurs at the top or the tip of the root.

**//__DESIGN of investigation__//**
//(complete/2; partial/1; not done appropriately or at all/0)

Are more cells in mitosis or interphase at the top of the root or at the tip of the root? Independent (conditions)** top and tip of root When counting, size of root tip and root top area is the same measure size of cells you are counting
 * __Aspect 1.__ Research question**//**:**
 * //Variables://
 * Dependent (results)** number of cell in mitosis and in interphase at top and tip of root
 * Control** (**constant)** keep the garlic bulbs in the same conditions so that the tops and tips of the roots are exposed to the same water and temperature.


 * Theory.** Since roots grow through mitosis, more cells in mitosis should be found where the most growth is occurring. Root growth can also occur when cells elongate ( grow larger).....

Garlic clove, 2 tooth picks, boiling tube (large testtube)
 * //__Aspect 3__ Method for collection of data//:**

Per lab table: Fill test tube with water. Submerge the apex of a garlic clove 1 cm into the water of the test tube. Keep the rest of the garlic clove above the water by inserting two tooth picks into the clove and balance across the top of the test tube. Leave undisturbed in a warm place for 48 hours. Per student: Choose a root from your table's garlic clove.

Follow directions from Robert's Biology student's manual: p 221, steps 1 - 9. Note that the stain will be already prepared for you and that each stage of mitosis does not need to be differentiated for this lab. You need to differentiate mitosis and intephase, as stated below. //**To record data:**// Count the cells in mitosis and those in interphase for the root top and the root tip Measure the size of three cells in the root top and in the root tip

The garlic bulb is placed at the top of a boiling test tube filled with water so that the bottom of the bulb is submerged 1 cm. This ensures that the root top and root tip will be both exposed to surrounding water. Water will be added if the level drops. The water temperature is the same throughout since the test tubes are all in the same environment.
 * //__Aspect 2__ Method for controlling the variables//**

**__//DATA Collection and Processing//__**
(complete/2; partial/1; Not done appropriately or at all/0

//**__Aspect 1__:**// - Qualitative record Diagram a section of the plant tip or root and show how you identified mitosis or interphase

1. Record quantitative count of cells in mitosis and cells in interphase for top of root and tip of root 2. Repeat each count 3 times in order to esatablish quantitative uncertainty. 3. Record data from other students so you have 3 data sets 4. Record the sizes of 3 cells from the top and from the tip of the root
 * //- Quantitative record .// Don't forget to include units and uncertainties where relaevant; for example, is each cell clearly distinguishable between mitosis or interphase?**

5. Calculate the uncertainty for your data by +/- 6.. Calculate the mean of the 3 data sets 7. Calculate the mean of each of your 3 cell sizes 8. Graph the mean values for root tip and for root tip 7. Include relevant error and uncertainty in both
 * //__Aspect 2__: Processing data//**
 * //Error and uncertainties//**

//**__Conclusion and Evaluation__**//
(c/2; p/1; n/0) Answer the research question Type in the content of your new page here.